Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.05-0.3 ng/ml.
Protein Construction
A DNA sequence encoding the canine FGF1 (XP_849274.1) (Phe16-Asp155) was expressed with a N-terminal Met.
Accession#
XP_849274.1
Expressed Host
E. coli
Species
Canine
Predicted N Terminal
Met
Molecule Mass
The recombinant canine FGF1 comprises 141 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 16 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.