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Project Description

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FGF-21 Protein, Human, Recombinant (His Tag)

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Specification

Cat. No.

NSC1051

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

FGF-21 Protein, Human, Recombinant (His Tag)

Size

50 µg

Price

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Synonyms

UNQ3115 / PRO10196 Protein, Human

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-1 µg/mL in the presence of 2.5 µg/mL of rmKlotho β.

Protein Construction

A DNA sequence encoding the mature form of human FGF21 (NP_061986.1) (His 29-Ser 209) was expressed, with a polyhistide tag at the N-terminus.

Accession#

NP_061986.1

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human FGF21 consisting of 188 amino acids and has a calculated molecular mass of 20.2 kDa. It migrates as an 23 kDa band in SDS-PAGE under reducing conditions as predicted.

Formulation

Lyophilized from 50 mM Tris, 10 % glycerol, 0.05 % Brij 35, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.