≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin
< 5 EU per mg of the protein.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The EC50 for this effect is typically 3-30 ng/mL.
Protein Construction
A DNA sequence encoding the mature form of human FGF10 (NP_004456.1) (Gln38-Ser208) was expressed.
Accession#
NP_004456.1
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human FGF10 consists of 172 amino acids and has a predicted molecular mass of 19.46 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.