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Project Description

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Ephrin B2 Protein, Mouse, Recombinant (His Tag)

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Specification

Cat. No.

NSC057

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

Ephrin B2 Protein, Mouse, Recombinant (His Tag)

Size

100 µg

Price

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Synonyms

ELF-2 Protein, Mouse; Epl5 Protein, Mouse; Eplg5 Protein, Mouse; Htk-L Protein, Mouse; LERK-5 Protein, Mouse; Lerk5 Protein, Mouse; NLERK-1 Protein, Mouse

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Immobilized Ephrin B2 Protein, Mouse, Recombinant (His Tag) at 2 μg/mL (100 μL/well) can bind EphB4 Protein, Mouse, Recombinant (hFc Tag), the EC50 is 3-18 ng/mL.

Protein Construction

A DNA sequence encoding the mouse EFNB2 (NP_034241.2) extracellular domain (Met 1-Ala 232) was fused with a polyhistidine tag at the C-terminus.

Accession#

NP_034241.2

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Arg 29

Molecule Mass

The secreted recombinant mouse EFNB2 comprises 215 amino acids and has a predicted molecular mass of 23.5 kDa. As a result of glycosylation, the apparent molecular mass of rmEFNB2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.