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Project Description

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Ephrin B2 Protein, Human, Recombinant

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Specification

Cat. No.

NSC054

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

Ephrin B2 Protein, Human, Recombinant

Size

100 µg

Price

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Synonyms

EPLG5 Protein, Human; Htk-L Protein, Human; HTKL Protein, Human; LERK5 Protein, Human

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1.Measured by its binding ability in a functional ELISA. 2.Immobilized human EFNB2 at 10μg/mL (100μL/well) can bind biotinylate human EphB4-Fc, the EC50 of biotinylate human EphB4-Fc is 5-60 ng/mL.

Protein Construction

A DNA sequence encoding the human EFNB2 (NP_004084.1) (Met1-Ala229) was expressed with six amino acids (ENLYFQ) at the C-terminus was expressed and purified.

Accession#

NP_004084.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Ile 28

Molecule Mass

The recombinant human EFNB2 consists of 209 amino acids and predicts a molecular mass of 23.1 KDa. It migrates as an approximately 32-36 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.