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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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Ephrin B2 Protein, Canine, Recombinant (His Tag)

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Specification

Cat. No.

NSC060

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

Ephrin B2 Protein, Canine, Recombinant (His Tag)

Size

100 µg

Price

Inquiry

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized canine EFNB2-His at 10 μg/ml (100 μl /well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 9-20 ng/ml.

Protein Construction

A DNA sequence encoding the canine EFNB2 (NP_001107768.1) (Met1-Ala229) was expressed with a C-terminal polyhistidine tag.

Accession#

NP_001107768.1

Expressed Host

HEK293 Cells

Species

Canine

Predicted N Terminal

Ile 28

Molecule Mass

The recombinant canine EFNB2 comprises 213 amino acids and has a predicted molecular mass of 23.6 kDa. The apparent molecular mass of the protein is approximately 33-38 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.