Ephrin B2 Protein, Canine, Recombinant (His Tag)

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Ephrin B2 Protein, Canine, Recombinant (His Tag)

Specification
Cat. No.
NSC060
Category
Neural Stem Cell Differentiation Markers
Product Type
Proteins
Product Name
Ephrin B2 Protein, Canine, Recombinant (His Tag)
Size
100 µg
Price
Inquiry
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA. Immobilized canine EFNB2-His at 10 μg/ml (100 μl /well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 9-20 ng/ml.
Protein Construction
A DNA sequence encoding the canine EFNB2 (NP_001107768.1) (Met1-Ala229) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_001107768.1
Expressed Host
HEK293 Cells
Species
Canine
Predicted N Terminal
Ile 28
Molecule Mass
The recombinant canine EFNB2 comprises 213 amino acids and has a predicted molecular mass of 23.6 kDa. The apparent molecular mass of the protein is approximately 33-38 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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