Inquiry

* Name

Phone

* Email

* Services or Products of Interest

Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

* Tick to confirm the Research Use Only (RUO) statement.

EGF Protein, Rat, Recombinant

Inquiry

Specification

Cat. No.

NSC885

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

EGF Protein, Rat, Recombinant

Size

100 µg

Price

Inquiry

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.05-0.3ng/mL.

Protein Construction

A DNA sequence encoding the rat EGF (NP_036974.1) (Asn974-Arg1026) was expressed and purified with an initial Met.

Accession#

NP_036974.1

Expressed Host

E. coli

Species

Rat

Predicted N Terminal

Met

Molecule Mass

The recombinant rat EGF consists of 54 amino acids and has a predicted molecular mass of 6.3 kDa. The apparent molecular mass of the recombinant protein is approximately 6-9 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

EGFR Signaling Pathway Jak-Stat Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.