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Project Description

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EGF Protein, Human, Recombinant (ECD), HPLC-verified

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Specification

Cat. No.

NSC880

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

EGF Protein, Human, Recombinant (ECD), HPLC-verified

Size

100 µg

Price

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Synonyms

Epidermal Growth Factor Protein, Human; HOMG4 Protein, Human; URG Protein, Human

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Endotoxin

< 5 EU per mg of the protein.

Activity

1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 (Cat#10014-HNAE), VEGFA (Cat# 11066-HNAH), EGF (Cat#10605-HNAE). Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested)2.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml.

Protein Construction

A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn971-Arg1023) was expressed and purified with an initial Met at the N-terminus.

Accession#

NP_001954.2

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.35 kDa as estimated in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

EGFR Signaling Pathway Jak-Stat Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.