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Project Description

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EGF Protein, Human, Recombinant (ECD, hFc Tag)

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Specification

Cat. No.

NSC879

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

EGF Protein, Human, Recombinant (ECD, hFc Tag)

Price

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Synonyms

Epidermal Growth Factor Protein, Human; HOMG4 Protein, Human; URG Protein, Human

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL.

Protein Construction

A DNA sequence encoding the mature soluble form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was fused with the Fc region of human IgG1 at the N-terminus.

Accession#

NP_001954.2

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu

Molecule Mass

The recombinant human Fc/EGF chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 290 amino acids and has a calculated molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/EGF monomer is approximately 37 kDa due to the glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

EGFR Signaling Pathway Jak-Stat Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.