EGF Protein, Human, Recombinant (ECD, hFc Tag)

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EGF Protein, Human, Recombinant (ECD, hFc Tag)

Specification
Cat. No.
NSC879
Category
Neural Stem Cell Growth Factors
Product Type
Proteins
Product Name
EGF Protein, Human, Recombinant (ECD, hFc Tag)
Price
Inquiry
Synonyms
Epidermal Growth Factor Protein, Human; HOMG4 Protein, Human; URG Protein, Human
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL.
Protein Construction
A DNA sequence encoding the mature soluble form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was fused with the Fc region of human IgG1 at the N-terminus.
Accession#
NP_001954.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu
Molecule Mass
The recombinant human Fc/EGF chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 290 amino acids and has a calculated molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/EGF monomer is approximately 37 kDa due to the glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Related Pathways
EGFR Signaling Pathway Jak-Stat Signaling Pathway
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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