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Project Description

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EGF Protein, Human, Recombinant (ECD), GMP grade, HPLC-verified

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Specification

Cat. No.

NSC878

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

EGF Protein, Human, Recombinant (ECD), GMP grade, HPLC-verified

Size

100 µg

Price

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Synonyms

Epidermal Growth Factor Protein, Human; HOMG4 Protein, Human; URG Protein, Human

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Endotoxin

< 5 EU per mg of the protein.

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The specific activity of Recombinant Human EGF is >1,000 x IU/μg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).

Protein Construction

A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified with an initial Met at the N-terminus.

Accession#

NP_001954.2

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human EGF consists of 54 amino acids and has a calculated molecular mass of 6.35 kDa.

Formulation

Supplied as sterile PBS, pH 7.4Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping

Liquid. It is shipped out with blue ice.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Related Pathways

EGFR Signaling Pathway Jak-Stat Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.