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Project Description

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EGF-L6 Protein, Human, Recombinant (His & FLAG Tag)

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Specification

Cat. No.

NSC902

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

EGF-L6 Protein, Human, Recombinant (His & FLAG Tag)

Size

100 µg

Price

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Synonyms

MAEG Protein, Human; W80 Protein, Human

Purity

> 65 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10E4 cells/well areadded to CD4-coated plates (1.25μg/mL and 100μL/well),approximately 40%-60% will adhere specifically after 30 minutes at 37℃.

Protein Construction

A DNA sequence encoding the human EGFL6 (NP_056322.2) (Asn22-Asp553) was expressed with the flag tag at the N-terminus and the histidine tag at the C-terminus.

Accession#

NP_056322.2

Expressed Host

Baculovirus-insect cells

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human EGFL6 consists of 551 amino acids and has a calculated molecular mass of 61.5 kDa.

Formulation

Supplied as sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping

Liquid. It is shipped out with blue ice.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.