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Project Description

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DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His Tag)

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Specification

Cat. No.

NAE009

Neurotransmitter Associated Enzymes

DOPA Decarboxylase/DDC

Product Type

Proteins

Product Name

DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His Tag)

Size

100 µg

Price

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Synonyms

Aadc Protein, Mouse

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the mouse DDC (O88533) (Met 1-Glu 480) was expressed，with a C-terminal polyhistidine tag.

Accession#

O88533

Expressed Host

Baculovirus-Insect Cells

Species

Mouse

Predicted N Terminal

Met

Molecule Mass

The recombinant mouse DDC consists of 490 amino acids and has a calculated molecular mass of 55.2 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 3mM DTT. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.