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Project Description

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DOPA Decarboxylase/DDC Protein, Human, Recombinant (His Tag), HPLC-verified

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Specification

Cat. No.

NAE008

Neurotransmitter Associated Enzymes

DOPA Decarboxylase/DDC

Product Type

Proteins

Product Name

DOPA Decarboxylase/DDC Protein, Human, Recombinant (His Tag), HPLC-verified

Size

50 µg

Price

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Synonyms

AADC Protein, Human

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.

Protein Construction

A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.

Accession#

NP_000781.1

Expressed Host

Baculovirus-Insect Cells

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human DDC consists of 490 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.

Formulation

Supplied as sterile 50mM Tris, 100mM NaCl, pH 8, 10% glycerol. Please contact us for any concerns or special requirements.. Please refer to the specific buffer information in the hard copy of CoA.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.