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Project Description

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CD166/ALCAM Protein, Rat, Recombinant (hFc Tag)

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Specification

Cat. No.

NSC552

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

CD166/ALCAM Protein, Rat, Recombinant (hFc Tag)

Size

100 µg

Price

Inquiry

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Immobilized rat CD6-His at 10 μg/ml (100 μl/well) can bind rat ALCAM-Fc3, The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml.

Protein Construction

A DNA sequence encoding the rat ALCAM (O35112) (Met1-Lys527) was expressed with the Fc region of human IgG1 at the C-terminus.

Accession#

O35112

Expressed Host

HEK293 Cells

Species

Rat

Predicted N Terminal

Trp 28

Molecule Mass

The recombinant rat ALCAM/Fc is a disulfide-linked homodimer. The reduced monomer comprises 741 amino acids and has a predicted molecular mass of 83.1 kDa. The apparent molecular mass of the protein is approximately 94 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.