CD166/ALCAM Protein, Rat, Recombinant (hFc Tag)

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CD166/ALCAM Protein, Rat, Recombinant (hFc Tag)

Specification
Cat. No.
NSC552
Category
Neural Stem Cell Growth Factors
Product Type
Proteins
Product Name
CD166/ALCAM Protein, Rat, Recombinant (hFc Tag)
Size
100 µg
Price
Inquiry
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Immobilized rat CD6-His at 10 μg/ml (100 μl/well) can bind rat ALCAM-Fc3, The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml.
Protein Construction
A DNA sequence encoding the rat ALCAM (O35112) (Met1-Lys527) was expressed with the Fc region of human IgG1 at the C-terminus.
Accession#
O35112
Expressed Host
HEK293 Cells
Species
Rat
Predicted N Terminal
Trp 28
Molecule Mass
The recombinant rat ALCAM/Fc is a disulfide-linked homodimer. The reduced monomer comprises 741 amino acids and has a predicted molecular mass of 83.1 kDa. The apparent molecular mass of the protein is approximately 94 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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