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Project Description

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CD166/ALCAM Protein, Mouse, Recombinant (His Tag)

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Specification

Cat. No.

NSC550

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

CD166/ALCAM Protein, Mouse, Recombinant (His Tag)

Size

100 µg

Price

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Synonyms

AI853494 Protein, Mouse; BEN Protein, Mouse; CD166 Protein, Mouse; DM-GRASP Protein, Mouse; MuSC Protein, Mouse; SC1 Protein, Mouse

Purity

> 98 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Immobilized mouse ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.08-0.18 μg/ml.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Lys 527) of mouse ALCAM (NP_033785.1) precursor was expressed, fused with a polyhistidine tag at the C-terminus.

Accession#

NP_033785.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Trp 28

Molecule Mass

The recombinant mouse ALCAM consists of 511 amino acids and has a predicted molecular mass of 57.7 kDa. Due to glycosylation, rmALCAM migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.