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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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CD166/ALCAM Protein, Mouse, Recombinant (His & hFc Tag)

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Specification

Cat. No.

NSC549

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

CD166/ALCAM Protein, Mouse, Recombinant (His & hFc Tag)

Size

100 µg

Price

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Synonyms

AI853494 Protein, Mouse; BEN Protein, Mouse; CD166 Protein, Mouse; DM-GRASP Protein, Mouse; MuSC Protein, Mouse; SC1 Protein, Mouse

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Immobilized mouse CD6-His at 10 μg/ml (100 μl/well) can bind mouse ALCAM-Fc2h, The EC50 of mouse ALCAM-Fc2h is 0.12-0.28 μg/ml.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Lys 527) of mouse ALCAM (NP_033785.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Accession#

NP_033785.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Trp 28

Molecule Mass

The recombinant mouse ALCAM/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 748 amino acids and has a predicted molecular mass of 84.2 kDa. Due to glycosylation, in SDS-PAGE under reducing conditions, rmALCAM/Fc migrates with approximately molecular mass of 105-110 kDa & 210 kDa corresponding to the monomer and homodimer.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.