< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to cleave Butyrylthiocholine
. The specific activity is > 70 nmol/min/μg
.
Protein Construction
A DNA sequence encoding the mouse BCHE (NP_033868.3) (Met 1-Leu 603) was expressed, with a C-terminal polyhistidine tag.
Accession#
NP_033868.3
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
His 28
Molecule Mass
The secreted recombinant mouse BCHE consists of 587 amino acids and has a calculated molecular mass of 66.7 kDa. The apparent molecular mass of the recombinant protein is approximately 60-70 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 25mM Tris, 100mM NaCl, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.