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Project Description

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Butyrylcholinesterase Protein, Human, Recombinant (His Tag)

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Specification

Cat. No.

NAE002

Neurotransmitter Associated Enzymes

Butyrylcholinesterase

Product Type

Proteins

Product Name

Butyrylcholinesterase Protein, Human, Recombinant (His Tag)

Size

50 µg

Price

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Synonyms

CHE1 Protein, Human; CHE2 Protein, Human; E1 Protein, Human

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to cleave Butyrylthiocholine . The specific activity is >50,000 pmoles/min/μg .

Protein Construction

A DNA sequence encoding the human BCHE (NP_000046.1) (Met 1-Leu 602) was expressed, fused with a polyhistidine tag at the C-terminus.

Accession#

NP_000046.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu 29

Molecule Mass

The recombinant human BCHE consists of 585 amino acids and has a predicted molecular mass of 66.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh BCHE is approximately 85-95 kDa due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.