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Project Description

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BMP-2 Protein, Danio rerio (zebrafish), Recombinant

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Specification

Cat. No.

NSC805

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

BMP-2 Protein, Danio rerio (zebrafish), Recombinant

Size

20 µg

Price

Inquiry

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 0.5-3 μg/mL.

Protein Construction

A DNA sequence encoding the zebrafish BMP2A (B3DI86) (Gln272-Arg386) was expressed and purified with an initial Met.

Accession#

B3DI86

Expressed Host

E. coli

Species

Danio rerio (zebrafish)

Predicted N Terminal

Met

Molecule Mass

The recombinant zebrafish BMP2A consists of 116 amino acids and has a calculated molecular mass of 13.2 kDa. It migrates as an 13 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 30mM HAC, pH 3.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.