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Project Description

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Betacellulin Protein, Mouse, Recombinant (His & hFc Tag)

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Specification

Cat. No.

NSC870

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

Betacellulin Protein, Mouse, Recombinant (His & hFc Tag)

Size

100 µg

Price

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Synonyms

Bcn Protein, Mouse

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 ng/mL.

Protein Construction

A DNA sequence encoding the mouse BTC (NP_031594.1) extracellular domain (Met 1-Gln 118) was fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag.

Accession#

NP_031594.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Asp 32

Molecule Mass

The recombinant mouse BTC/Fc is a disulfide-linked homodimer. The reduced monomer consists of 335 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of rm BTC/Fc monomer migrates with an apparent molecular mass of 50-55 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.