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Project Description

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Betacellulin Protein, Human, Recombinant (hFc Tag)

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Specification

Cat. No.

NSC869

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

Betacellulin Protein, Human, Recombinant (hFc Tag)

Size

100 µg

Price

Inquiry

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-40 ng/mL.

Protein Construction

A DNA sequence encoding the human BTC (P35070) (Met1-Tyr111) was expressed with the Fc region of human IgG1 at the C-terminus.

Accession#

P35070

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Asp 32

Molecule Mass

The recombinant human BTC/Fc is a disulfide-linked homodimer. The reduced monomer comprises 321 amino acids and has a predicted molecular mass of 36 kDa. The apparent molecular mass of the protein is approximately 43-46 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.