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Project Description

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beta-Catenin Protein, Human, Recombinant (His & GST Tag)

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Specification

Cat. No.

NSC1357

Category

Neural Stem Cell Markers

Product Type

Proteins

Product Name

beta-Catenin Protein, Human, Recombinant (His & GST Tag)

Size

100 µg

Price

Inquiry

Synonyms

armadillo Protein, Human; CTNNB Protein, Human; MRD19 Protein, Human

Purity

> 85 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the human CTNNB1 (P35222-1) (Met 1-Leu 781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Accession#

P35222-1

Expressed Host

Baculovirus-Insect Cells

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.

Formulation

Supplied as sterile 50mM Tris, 150mM NaCl, 25% glycerol, pH 8.0, 0.1mM EDTA, 1mM TCEP, 0.4mM PMSF, 0.5mM GSHPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping

Liquid. It is shipped out with blue ice.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

Canonical Wnt Pathway EGFR Signaling Pathway Non-Canonical Wnt Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.