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Project Description

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Acetylcholinesterase Protein, Mouse, Recombinant (His Tag)

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Specification

Cat. No.

NAE001

Neurotransmitter Associated Enzymes

Acetylcholinesterase

Product Type

Proteins

Product Name

Acetylcholinesterase Protein, Mouse, Recombinant (His Tag)

Size

20 µg

Price

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Synonyms

Acre Protein, Mouse; Chrne Protein, Mouse

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to cleave Acetylthiocholine.The specific activity is > 250 nmols/min/μg.

Protein Construction

A DNA sequence encoding the mouse ACHE (NP_033729.1) (Met 1-Leu 614) was expressed, with a polyhistidine tag at the C-terminus.

Accession#

NP_033729.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Glu 32

Molecule Mass

The recombinant mouse ACHE consists of 594 amino acids and has a predicted molecular mass of 66.2 kDa as estimated in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.