Introduction

Humoral cytokine and chemokine analysis has become pivotal in the diagnosis, classification, and evaluation of inflammatory diseases. In ophthalmology, ocular allergies, dry eye disease (DED) and uveitis are the most common ocular inflammatory conditions. Ocular cells that can produce cytokines include the retinal pigment epithelium (RPE), Müller cells, lens epithelium, corneal stromal and epithelium, and ciliary body epithelium. Studies have shown that cytokines and chemokines form a coherent view of the role of inflammation in eye disease. Their use as potential biomarkers can help further support the discovery and development of new drugs and the establishment of personalized medicine. Ace Therapeutics' scientists support project development for customers around the world using flow cytometry to assess cytokines in ocular matrices such as tears.

Fig. 1. The role of TNF-α and IL-10 in the ocular inflammation and dry eyes. (Gao F, et al., 2021)

Our Services

As a comprehensive ophthalmic solutions company, Ace Therapeutics is focused on discovering new and rare cell types through multiparametric analysis. This facilitates more detailed characterization of cell populations and the identification of subpopulations as markers of disease. On this basis, cell biomarker workflows are further supported by providing pure populations for genomic and proteomic analyses, allowing simple and reliable isolation of these cells for downstream analysis.

Our scientific experts and biomarker laboratory staff can help you develop a translational design strategy to identify, develop assays, and screen multiple biomarkers in parallel. This will help you to get you to a proof of concept.

What Can We Provide?

At Ace Therapeutics, we provide clients with direct expression and comparative analysis of biomarkers using multicolor fluorescence flow cytometry.

To the satisfaction of our customers, our scientists can use our advanced flow cytometers to simultaneously analyze multiple cell specific biomarkers and cell viability biomarkers in single cell level analysis, and the detection effect is extremely sensitive, not only The compensation is minimal, and there is substantial flexibility in the choice of fluorochromes. In addition, high-throughput rapid analysis of samples can also be performed, and up to four different fluorescent dye-labeled biomarkers can be analyzed simultaneously.

Our Experience

• Rare Cell Analysis

Rare cells are low abundance cells among a large number of background cells. Ace Therapeutics has developed a flexible platform for highly sensitive and specific analysis of rare cells. It can accommodate fixed or living cell samples, enabling deep phenotyping and highly customized analysis of ocular cell biomarkers.

• Primary Cell Culture

Ace Therapeutics provides primary cell isolation and culture services to support researchers in the discovery and analysis of biomarkers. Primary cell cultures are capable of generating more biologically relevant data than cell lines. They can be used in the development of tests and the identification of ocular diagnostic and prognostic biomarkers.

• Flow Cytometry

Flow cytometry is a highly versatile method  for the discovery of novel biomarkers. Ace Therapeutics uses flow cytometry for immunophenotyping, cell counting, and assessment of many cellular functions, such as intracellular cytokine production, protein phosphorylation, cell proliferation, and apoptosis.

• Cell-Based Assays

Ace Therapeutics also offers customers a full range of cell-based assays  services. Our cell-based assays can be used to test cytotoxicity, or to determine drug potency and mechanism of action.

Our Advantages

• Rich experience in cytology and biomarker analysis.
• Unique comprehensive service for ocular biomarker research.
• Excellent research team.
• Comprehensive research platforms.

Ace Therapeutics has the most qualified team to serve global customers. Our scientists are always ready to provide high-quality ocular cell biomarker discovery and analysis solutions to global customers. If you have questions about the content of this service, please feel free to contact us. We look forward to hearing from you.

Reference

1. Gao F, Hong X, Ding F, et al. High Level of Inflammatory Cytokines in the Tears: A Bridge of Patients with Concomitant Exotropia and Dry Eye. Oxid Med Cell Longev. 2021;2021:5662550.

• Proteomic-Based Ocular Biomarker Discovery and Analysis Services
• Genomics-Based Ocular Biomarker Discovery and Analysis Services
• Metabolomics-Based Ocular Biomarker Discovery and Analysis Services
• Epigenomic-based Ocular Biomarkers Discovery and Analysis Services
• Lipidomics-Based Ocular Biomarker Discovery and Analysis Services
• Ocular Tissue Biomarker Discovery and Analysis Services

• * Name:
• Phone:
• * Email:
• * Services or Products of Interest:
• Project Description:
• * All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

  * Tick to confirm the Research Use Only (RUO) statement.

• Submit