Age-related macular degeneration (AMD) is a predominant cause of vision loss in older adults, significantly impacting quality of life. Understanding the complexities of AMD requires sophisticated models that can accurately recapitulate the human disease. Zebrafish models have emerged as a powerful tool, offering unique advantages in mimicking the human retinal environment and facilitating the understanding of AMD mechanisms. Ace Therapeutics specializes in providing preclinical services to develop zebrafish models, providing critical insights into AMD research and discovery of potential therapeutics.

The Role of Zebrafish Models in AMD Research

AMD primarily presents in two forms: atrophic (dry) AMD and neovascular (wet) AMD. The atrophic form is marked by geographic atrophy of the retinal pigment epithelium (RPE) and surrounding photoreceptors, while the neovascular form involves the growth of abnormal blood vessels beneath the retina. The interplay of genetic, environmental, and age-related factors complicates the pathology of AMD.

Zebrafish (Danio rerio) have become a valuable model for studying AMD due to their cone-rich retina, which closely resembles that of humans. Their diurnal behavior and transparent embryos allow for real-time observation of retinal development and disease progression. These unique features make it possible to observe retinal structure and function using non-invasive imaging techniques.

Fig. 1 . Comparison of the human and zebrafish retina. (Noel NCL, et al., 2022)

Service Overview

Ace Therapeutics specializes in developing customized zebrafish models specifically for AMD research. By utilizing genetic engineering techniques, such as CRISPR/Cas9, we develop models that recapitulate the genetic and phenotypic characteristics of AMD. Our models can incorporate various genetic mutations associated with AMD, allowing for the exploration of disease mechanisms and the development of potential therapeutic interventions.

Our comprehensive services encompass model design, phenotyping, and preclinical evaluation, providing robust and reproducible research outcomes. The combination of expertise in zebrafish genome-editing and cutting-edge technology positions Ace Therapeutics as a leader in preclinical AMD research.

Explore Ace Therapeutics' Zebrafish Models of AMD

At Ace Therapeutics, we specialize in delivering customized zebrafish models tailored specifically for AMD research. Our extensive range of services is designed to facilitate the investigation of AMD mechanisms and the evaluation of potential therapeutics.

• Transgenic Models
  Using genetic engineering techniques such as CRISPR/Cas9, we develop transgenic zebrafish that expresses specific genetic mutations associated with AMD. This method allows for precise manipulation of genes such as CFH and EYS, which are implicated in the disease. By introducing these mutations, we can recapitulate the genetic predispositions seen in human AMD.
• Environmental Stress Models
  We also develop AMD models induced by exposure to high-fat diets, oxidative stress, and light exposure. These models are crucial for studying the interplay between genetic susceptibility and environmental triggers, allowing researchers to evaluate how these factors influence disease progression.

Drug Screening Assays with Zebrafish Models

Our zebrafish models are equipped for high-throughput drug screening, enabling the evaluation of potential drug candidates. We utilize techniques such as microinjections for drug delivery and assess their effects on retinal health through various endpoints, including visual function tests and histological analyses.

Quantitative Analysis of Vascular Changes

Employing transgenic lines with fluorescently labeled blood vessels enables the quantification of vascular changes, such as neovascularization and vascular regression, which are critical in AMD.

Ace Therapeutics is dedicated to providing high-quality zebrafish models to support in-depth preclinical research into the mechanisms of AMD. Zebrafish offers unique advantages for in vivo imaging and genetic manipulation, facilitating studies of AMD progression and drug discovery. For more information about our zebrafish models and how we can assist you in your AMD research, please do not hesitate to contact us.

Reference

1. Noel NCL, et al. Zebrafish and inherited photoreceptor disease: Models and insights. Prog Retin Eye Res. 2022;91:101096.

• By Ocular Disease Type
• By Zebrafish Modeling Method