Customized Rodent Models of Retinal Vein Occlusion

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Transgenic mice offer unparalleled possibilities to study innate immunity and molecular biology cascades during retinal vein occlusion (RVO). Ace Therapeutics researchers successfully establish a mouse model of retinal vein occlusion. This model helps our clients study retinal diseases characterized by retinal edema and ischemia-hypoxia. Alternatively, the nervous system is associated with vascular injury, neuronal death, and inflammatory processes. Here, our highly qualified scientists work closely with you every step of the way to provide the highest quality and most convenient service and support.

Rodent Models of Retinal Vein Occlusion

What Is Retinal Vein Occlusion?

Retinal vein occlusion (RVO) is the second most common retinal vascular disease leading to vision loss. RVO can be classified as branch retinal vein occlusion (BRVO) or central retinal vein occlusion (CRVO) depending on the site of occurrence. Both can lead to capillary detachment, or widespread ischemia within the retina, eventually leading to vision loss. The degree of vision loss and retinal ischemia depends on the severity and location of the obstruction. RVO pathological features include retinal edema, hypoxic-ischemic injury, and inflammation. Currently, there is no clinically effective treatment for RVO patients. Therefore, robust animal models are needed to study disease mechanisms and to test the efficacy and potential toxicity of novel therapies or to develop improved treatment therapies.

Fig. 1. Timeline and schematic of the RVO mouse model. (Colón Ortiz C, et al., 2021)

Service Overview

The experimental model based on retinal vein laser photocoagulation is the most widely used means to induce the retinal vein occlusion model. Ace Therapeutics has successfully developed and validated a mouse model of retinal vein occlusion (RVO) using intravenous laser photocoagulation, which helps customers study the pathogenesis of hypoxic-ischemic injury in the neural retina. Importantly, our talented ophthalmic pharmacology scientists have also developed experimental protocols to test the therapeutic intervention of novel treatments or compounds on retinal degeneration in this model.

Explore the Ace Therapeutics' Rodent Models of Retinal Vein Occlusion

Ace Therapeutics' retinal vein occlusion model involves intravenous injection of a photosensitizer dye and laser photocoagulation using a retinal imaging microscope attached to an ophthalmology-guided laser.

Specifically, photosensitization by injecting the light-sensitive dye rose bengal into the tail vein of C57Bl/6J mice, followed by laser treatment of large 3 different retinal veins in one eye, thereby precisely and robustly inducing retinal vein occlusion model formation. The predominant morphological pathology of RVO in this model was retinal edema. Our investigators assess the degree of retinal edema at various time points by optical coherence tomography (OCT) measuring the longitudinal retinal thickness in the occluded area over a follow-up period of up to eight days.

Available Support Services

To characterize the success of model building and test the potential efficacy of therapeutic drugs, Ace Therapeutics also provides the following supporting endpoint evaluation services, including but not limited to:

Optical coherence tomography (OCT)
Fundoscopy with the Micron III system
Fluorescein angiography
Histological evaluation
ELISA, Western blotting, qPCR

Ace Therapeutics' rodent models of retinal vein occlusion model have been verified by a large number of experiments, which can accelerate your research on retinal diseases. With the rich experience accumulated in completing multiple projects, we are confident to be your most reliable partner. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Colón Ortiz C, Potenski A, Lawson JM, et al. Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability. J Vis Exp. 2021, 174:10.3791/62980.
Khayat M, Lois N, Williams M, et al. Animal Models of Retinal Vein Occlusion. Invest Ophthalmol Vis Sci. 2017, 58(14):6175-6192.

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