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Customized Rodent Models of Retinal Ischemia-Reperfusion Injury

Rodent models of retinal ischemia-reperfusion (I/R) injury provide researchers with the opportunity to understand the pathogenesis and therapeutic strategies of I/R injury in humans. As a professional provider of live animal models of ocular diseases, Ace Therapeutics scientists have successfully developed rodent models of retinal ischemia-reperfusion injury for our customers to explore new therapies and explain therapeutic mechanisms. Here, our experienced researchers will generate an exclusive customized protocol for you according to your project.

Rodent Models of Retinal Ischemia-Reperfusion Injury

The Necessity of a Retinal Ischemia-Reperfusion Injury Model

Retinal ischemia-reperfusion (I/R) injury is a common cause of visual impairment. It is a feature of many common eye diseases such as glaucoma, age-related macular degeneration (AMD), diabetic retinopathy, and retinopathy of prematurity. It occurs through a reduction in blood supply to the retina, resulting in impaired delivery of oxygen and other nutrients to the individual retinal layers, leading to severe oxidative and inflammatory damage when circulation is restored (reperfusion). In particular, retinal ganglion cells (RGC) die. However, little is known about the overall I/R mechanism leading to retinal damage. To better understand the pathophysiological mechanisms associated with retinal I/R injury, the development of robust rodent models is needed to gain insight into its pathogenesis and explore potential strategies.

Fig. 1. I/R induced retinal detachment at day 3 and day 7 after injury. (Kim BJ, et al., 2013)

Service Overview

Pressure-induced retinal I/R injury is the most widely accepted protocol. Ace Therapeutics researchers successfully establish a rodent model of retinal ischemia-reperfusion (I/R) injury by raising intraocular pressure. This model helps our clients explore the mechanisms of human I/R injury and develop potential therapeutic strategies, especially targeting neurodegenerative damage to the retinal neurovascular unit. It is worth mentioning that our customers have used this model to study ischemia-induced ocular lesions such as glaucoma and diabetic retinopathy.

Explore the Ace Therapeutics' Rodent Models of Retinal Ischemia-Reperfusion (I/R) Injury

Ace Therapeutics' rodent model of retinal ischemia-reperfusion injury is achieved by elevating the saline container height to elevate IOP above systolic blood pressure.

Specifically, C57Bl/6J mice and SD rats were deeply anesthetized by intraperitoneal injection, and then a 30-gauge cannula (for mice) or a 32-gauge cannula (for rats) was inserted into the anterior chamber of the mice and rats, and injected with 0.9% Bacterial saline to produce retinal ischemia under elevated intraocular pressure. The needle is then released from the eye and intraocular pressure is restored, inducing a retinal ischemia-reperfusion model. Utility of this model for cellular injury and dysfunction, particularly neurodegeneration, in the neurovascular unit of the rodent retina. This results in a dramatic loss of vision and the death of retinal cells, which can be used to test the neuroprotective effects of potential compounds or new treatments.

Available Support Services

Ace Therapeutics' highly qualified scientists also develop standard procedural protocols to test potential therapeutic strategies and evaluate them in the following ways, including but not limited to:

Spectral-domain optical coherence tomography (SD-OCT) analysis
Flash scotopic electroretinogram (ERG) analysis
Quantitative analysis of retinal ganglion cells
Retinal thickness analysis (OCT)
Histopathological analysis
ELISA, Western blotting, qPCR
Bioinformatics

Ace Therapeutics will provide global customers with the most cost-effective and time-effective models of retinal ischemia-reperfusion injury. Our scientists take a hands-on approach, working side-by-side with clients to understand their specific preclinical research requirements. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Kim BJ, Braun TA, Wordinger RJ, et al. Progressive morphological changes and impaired retinal function associated with temporal regulation of gene expression after retinal ischemia/reperfusion injury in mice. Mol Neurodegener. 2013, 22;8:21.
Hartsock MJ, Cho H, Wu L, et al. A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure. J Vis Exp. 2016,113:54065.

For Research Use Only.

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