Customized Rodent Models of LPS-Induced Uveitis

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Uveitis is a group of ocular inflammatory diseases that can lead to permanent vision loss in severe cases. Ace Therapeutics provides our global customers with a reliable, simple, and reproducible protocol to induce rodent uveitis models. With our extensive experience in the field of uveitis, you have reason to trust that we can help you overcome any difficulties in your research path, thus facilitating the development of your project.

Rodent Models of LPS-Induced Uveitis

Anterior Uveitis and LPS-Induced Uveitis Model

Uveitis is a relatively common severe ocular inflammation of the uvea of unknown etiology. It represents a variety of intraocular inflammations, which may be related to complications of autoimmune diseases, viral infections, bacterial infections, chemical injury, and metabolic problems. Its clinical manifestations are eye pain, redness, photophobia, and decreased vision. Uveitis can be divided into anterior uveitis, intermediate uveitis, posterior uveitis, and panuveitis. Anterior uveitis, which consists of iridocyclitis and iritis, is caused by an inflammatory response inside the eye. In rodent models, endotoxin-induced uveitis (EIU) is an effective experimental model to study the complex pathogenesis associated with anterior uveitis and evaluate new drugs. Clinical symptoms are dominated by inflammatory exudates and infiltrating cells associated with inflammatory features.

Fig. 1. Retinal evaluation of the endotoxin-induced uveitis (EIU) model. (Zhang J, et al., 2022)

Service Overview

The lipopolysaccharide (LPS)-induced rodent uveitis model was used to mimic human anterior uveitis and test ocular novel anti-inflammatory therapies. With years of experience in drug development and model building in uveitis, Ace Therapeutics can provide a series of preclinical services for the development of uveitis ophthalmic products for global customers, including the development of anterior uveitis models, mechanism research, and evaluation of new therapies.

Explore the Ace Therapeutics' Rodent Models of LPS-Induced Uveitis

LPS-induced uveitis in rodents is an excellent animal model for studying uveitis and associated acute retinal inflammation. In order to help researchers accelerate their understanding of uveitis pathogenic mechanisms and screen for potential therapeutic targets. Ace Therapeutics has developed the following two LPS-induced uveitis rodent models.

LPS-induced uveitis mouse model
LPS-induced uveitis rats model

Ace Therapeutics researchers induce a mouse EIU model by giving C57BL/6J mice a single intraperitoneal injection of 5 mg/kg of LPS. Alternatively, the rat EIU model was induced by subcutaneously injecting 200 µg/kg of LPS into Wistar rats. Both models represent a type of transient uveal inflammation, with the inflammatory response peaking approximately 24 hours after LPS administration.

In order to ensure the reliability of the model construction and the accurate assessment of ophthalmic products efficacy, Ace Therapeutics researchers evaluate clinical features of ocular inflammation in both eyes by biomicroscopy (slit lamp) and measurement of cell infiltration and protein concentration in aqueous humor and vitreous 24 h after LPS injection.

Ace Therapeutics is committed to being your trusted partner in eye disease research. Our researchers are equipped to provide unrivaled custom solutions for uveitis models for your project development. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Yadav UCS, Ramana KV. Endotoxin-Induced Uveitis in Rodents. Methods Mol Biol. 2019;1960:161-168.
Zhang J, Wu J, Lu D, et al. Retinal Proteomic Analysis in a Mouse Model of Endotoxin-Induced Uveitis Using Data-Independent Acquisition-Based Mass Spectrometry. Int J Mol Sci. 2022 Jun 9;23(12):6464.

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