Customized Rodent Models of Corneal Wound Healing

Inquiry

The mouse corneal wound healing model is an excellent model system for wound repair research. Benefiting from our advanced technology and rich experience in ocular disease model development, Ace Therapeutics has developed two corneal wound healing models, mechanical injury and chemical injury, for our global customers to study the effect of candidate compounds or potential therapies on the corneal recovery mechanism of treatment. Our researchers have many years of experience in the development of ocular disease models. We are capable of providing the most advanced technical support and services for your project development.

Rodent Models of Corneal Wound Healing

What Is Corneal Wound Healing?

The cornea is the part of the eye exposed to the external environment and is most likely to be damaged by various physicochemical factors. Corneal tissue integrity is especially critical for clear vision. Eye injuries such as physical or chemical trauma or severe infection can cause permanent corneal damage, leading to clouding and loss of vision. Corneal wound healing is a complex process involving cell death, migration, proliferation, differentiation, and extracellular matrix remodeling. Therefore, understanding the mechanism of corneal wound healing is a crucial and effective way to develop accelerated and improved wound healing. Among animal models, the mouse model has become an ideal model for corneal wound healing research due to its unique eye structure.

Fig. 1. Cellular interactions during corneal repair. (Ljubimov AV, et al., 2015)

Service Overview

The mouse corneal wound healing model is an ideal model to study the wound repair process. This is because of its simple anatomy and easy access to a normal no vascular state. Ace Therapeutics scientists have developed two mouse corneal wound healing models: a mechanical (epithelial) injury model and a chemical (stromal) injury model. These two models elicit distinct repair responses, and they can be used to independently study the effects of drug candidates or new therapies on cell migration, proliferation, differentiation, barrier function, scar formation, reinnervation, and angiogenesis/lymphangiogenesis.

How Ace Therapeutics Created a Mouse Model of Corneal Wound Healing?

Under general anesthesia in C57BL/6 mice, researchers at Ace Therapeutics create mechanical damage by adding pressure to the center of the cornea using an Algerbrush II with a 0.5 mm burr. Alternatively, apply NaOH-soaked filter paper to the central cornea with forceps for 30 s to create a chemical injury. Throughout the modeling process, our researchers applied a fluorescein solution and confirmed the size of the corneal epithelial defect using a microscope GFP filter.

Mechanical damage model
Chemical injury model (Alkali burn model)

Our Capabilities

In order to fully characterize the establishment of corneal wound healing models and evaluate the efficacy of candidate substances in promoting corneal healing, Ace Therapeutics provides a series of supporting inspection services, including but not limited to:

Ocular exam (fluorescein stain and slit lamp)
Fundus photography
Histopathological studies
Morphological analysis
Flow cytometry

Corneal wound healing is critical for restoring the cornea's integrity and maintaining vision after a corneal injury. Ace Therapeutics can provide you with excellent corneal wound healing models to accelerate your research. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Pan P, Chan MF. Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries. Methods Mol Biol. 2021, 2193:149-158.
Chan MF, Werb Z. Animal Models of Corneal Injury. Bio Protoc. 2015, 5(13):e1516.
Ljubimov AV, Saghizadeh M. Progress in corneal wound healing. Prog Retin Eye Res. 2015, 49:17-45.

For Research Use Only.

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