Customized Rodent Model of MNU-Induced Retinal Degeneration

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MNU-induced retinal degeneration is another ideal model to simulate human retinal degenerative diseases, including age-related macular degeneration (AMD), retinal detachment, and retinitis pigmentosa (RP). As a comprehensive provider of ocular disease models, Ace Therapeutics has developed rodent models of MNU-induced retinal degeneration for our global customers to evaluate the efficacy of new therapies or candidate compounds, and to determine the therapeutic mechanism of the disease. Here, you will benefit from our team of experienced experts to accelerate your project development.

Rodent Model of MNU-Induced Retinal Degeneration

MNU-Induced Retinal Degeneration Models

Retinitis pigmentosa (RP) and age-related macular degeneration (AMD) are a group of photoreceptor degenerative diseases characterized by dysfunction and degeneration of photoreceptor cells leading to impaired vision and eventually blindness. Among them, RP is a genetic disease, and various related gene mutations have been found, mainly related to the phototransduction pathway. On the contrary, AMD development is mainly associated with various environmental factors, such as smoking, obesity, and vitamin deficiency. Rodent models of retinal degeneration have been studied for many years to understand photoreceptor cell death mechanisms. N-methyl-N-nitrosourea (MNU) has been shown to induce degeneration of the outer layer of the retina in rodents and is an effective animal model of photoreceptor degenerative disease. Its photoreceptor cell loss occurred within 7 days after MNU administration by apoptosis similar to human RP/AMD. This provides a valuable tool for elucidating the mechanism and exploring potential therapeutic approaches to RP and AMD.

Fig. 1. Retinal cell death after blue light exposure. Fig. 1. Histological characteristics of N-methyl-N-nitrosourea-induced retinal degeneration. (Zhang S, et al., 2018)

Service Overview

Using MNU-induced retinal degeneration is another attractive model for modeling photoreceptor degeneration in human retinal degenerative diseases, which is highly reproducible. In order to meet the needs of different researchers, Ace Therapeutics has developed an economical and standardized MNU-induced rodent retinal degeneration model to help our customers develop novel treatments for retinal degenerative diseases. The model provides an opportunity for our clients to evaluate the efficacy of novel therapies or candidate compounds and also to determine the therapeutic mechanisms of disease. During the entire cooperation process, you will benefit from our experienced researchers and high-standard model development services to accelerate your project development.

Explore the Ace Therapeutics' Rodent Model of MNU-Induced Retinal Degeneration

Ace Therapeutics' MNU-induced rodent retinal degeneration model was obtained after a single intraperitoneal injection of N-methyl-N-nitrosourea (MNU) in C57/BL mice or SD rats. It can simulate well age-related macular degeneration (AMD) and retinitis pigmentosa (RP) in humans. Throughout the model-building process, we monitor and evaluate in the following ways:

Histopathology
Outer retinal layer (ONL) thickness
Electroretinogram (ERG)
Optical coherence tomography (OCT)
Western blot analysis
Immunohistochemistry

Ace Therapeutics has accumulated rich experience and scientific data in retinal degeneration model construction. In order to better promote customers' preclinical project research, we can also provide pharmacodynamic evaluation solutions for ophthalmic products or novel therapies. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Chen YY, Liu SL, Hu DP, et al. N -methyl- N -nitrosourea-induced retinal degeneration in mice. Exp Eye Res. 2014 Apr;121:102-13.
Zhang S, Zhang S, Gong W, et al. Müller Cell Regulated Microglial Activation and Migration in Rats With N- Methyl-N -Nitrosourea-Induced Retinal Degeneration. Front Neurosci. 2018 Dec 3;12:890.

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