Advantages of Chicken Corneas for Studying Corneal Injuries

The cornea is a layered, transparent, avascular tissue that acts as a barrier to the external environment and serves as the primary refractive component of the eye. When the cornea is damaged by the outside world, which leads to a cascade of intercellular signaling and wound-healing responses. Corneal wound healing is a complex process involving cell death, migration, proliferation, differentiation, and extracellular matrix remodeling. Therefore, understanding the mechanism of corneal wound healing is a key and effective way to accelerate and improve wound healing. In animal models, the chicken is another biological model of concern for corneal wound healing research, and the chicken cornea has several advantages over rodent wound healing models: 1) Chicken eyes and corneas are much larger than rodents, making these eyes more suitable for experimental manipulation. 2) the intraocular lens in the chicken is much smaller than in the rodent model, allowing for delivery of intravitreal injection of compounds such as BrdU without complication. 3) The price is cheap.4) chicken corneas have a true Bowman's membrane and the corneal layers are proportional to the human cornea.

Fig. 1. Wound healing of embryonic corneas. (Spurlin JW 3rd, et al., 2013)

Service Overview

Ace Therapeutics' scientists develop a chick corneal injury model given the anatomical similarity of the chick cornea and humans.

This model is an excellent model system for studying wound healing, scar tissue formation, and reinnervation of sensory peripheral neurons. It can be used to assess the impact of drug candidates or new therapies on axon regeneration and cell signaling pathways that regulate wound healing. Furthermore, it could be used to elucidate the mechanisms of corneal fibrosis and provide an opportunity to test potential candidates for therapeutic interventions to prevent tissue fibrosis in adults.

Explore Ace Therapeutics' Chicken Models of Corneal Wound Healing

Ace Therapeutics use a micro-dissecting knife (30°angled micro-dissecting knife) to induce a linear corneal injury model in post-natal day 7 chicks.

Specifically, our researchers make an incision in the chick cornea, and the laceration went through the corneal epithelium, basement membrane, and anterior stroma. Then, prevent eye infection with 0.3% Moxifloxacin topical ophthalmic solution.

Available Support Services

In order to fully characterize the establishment of corneal wound healing models and evaluate the efficacy of candidate substances in promoting corneal healing, Ace Therapeutics provides a series of supporting inspection services, including but not limited to:

• Ocular exam (fluorescein stain and slit lamp).
• Fundus photography.
• Histopathological studies.
• Morphological analysis.
• Flow cytometry.
• Molecular biology.

Ace Therapeutics aims to provide a valuable tool to help our global customers study the corneal wound-healing response to injury and develop promising therapies. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

1. Spurlin JW 3rd, Lwigale PY. Wounded embryonic corneas exhibit nonfibrotic regeneration and complete innervation. Invest Ophthalmol Vis Sci. 2013, 54(9):6334-44.
2. Ritchey ER, Code K, Zelinka CP, et al. The chicken cornea as a model of wound healing and neuronal re-innervation. Mol Vis. 2011, 17:2440-54.
3. Wisely CE, Sayed JA, Tamez H, et al. The chick eye in vision research: An excellent model for the study of ocular disease. Prog Retin Eye Res. 2017, 61:72-97.

• Customized Chicken Models of Myopia
• Customized Chicken Models of Hyperopia
• Customized Chicken Models of Inherited Eye Disease
• Customized Chicken Models of Retinal Injury
• Customized Chick Embryo Models of Uveal Melanoma

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