GM-04
Primary mouse retinal ganglion cells (RGCs) are cells derived from mouse retina, particularly important in glaucoma because they are the cells that are damaged primarily by the disease. Preserving the retinal ganglion cells and their axons is necessary for sight.
Cell Type
Retinal ganglion cells
Animal Species
Mus musculus (Mouse)
Tissue Source
Retina
Disease
Normal
Biosafety
Primary Mouse Retinal Ganglion Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Cell activity
>85% (Viability by Trypan Blue Exclusion)
UOM
>5X104 in Basal Medium w/ 90% FBS, 10% DMSO
Growth Properties
Adherent
Cell Recovery
After receiving the cells, shake at 37℃ in a water bath until completely dissolved, transfer toa15ml centrifuge tube, add 3-5 times complete culture solution, 1000 rpm for 5 min, discard the supernatant, and place in a T25 flask for culture.
Medium
Neurobasal-A medium, B-27 Supplement (50X), 1% Penicillin-Streptomycin Solution.
Temperature
37℃
Condition
95% air, 5% carbon dioxide
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping Conditions
Dry Ice
RGC is not recommended for expanding or long-term cultures because of its lack of proliferative capacity.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.