GM-08
Primary human trabecular meshwork cells (HTMC) are endothelial-like cells located in a sponge-like connective tissue near the front of the eye. HTMC make layers of beams and regulate eye pressure by controlling drainage of fluid into tubes that flow into the bloodstream. Efflux is mediated by changes in contractility and tension of HTMC. The decrease in its fluid outflow leads to increased eye pressure and gradual loss of retinal ganglion cells, leading to glaucoma and irreversible blindness.
Cell Type
Trabecular meshwork cells
Animal Species
Homo sapiens (Human)
Tissue Source
Trabecular Meshwork
Disease
Normal
Biosafety
Primary Human Trabecular Meshwork Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
UOM
5X105 cells/cryovial
| Product | Format | Cat No. | |
|---|---|---|---|
| Cryopreserved Primary Human Trabecular Meshwork Cells | 1 Kit | GM-08-K | Inquiry |
| Cryopreserved Primary Human Trabecular Meshwork Cells | 1 Cryovial | GM-08-C | Inquiry |
| Proliferating Primary Human Trabecular Meshwork Cells | T-25 Flask | GM-08-T25 | Inquiry |
| Proliferating Primary Human Trabecular Meshwork Cells | T-75 Flask | GM-08-T75 | Inquiry |
| Proliferating Primary Human Trabecular Meshwork Cells | 96-Well Plate | GM-08-96W | Inquiry |
Growth Properties
Adherent
Cell Recovery
After receiving the cells, shake at 37℃ in a water bath until completely dissolved, transfer toa15ml centrifuge tube, add 3-5 times complete culture solution, 1000 rpm for 5 min, discard the supernatant, and place in a T25 flask for culture.
Medium
DMEM/F12+5% FBS +1% Trabecular Meshwork Cells growth supplement+1% Penicillin-Streptomycin Solution.
Temperature
37℃
Condition
95% air, 5% carbon dioxide
Storage
Freeze of the liquid nitrogen (90% FBS +10% DMSO).
Shipping Conditions
Dry Ice
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