Aqueous humor (AH) plays an important role in the pathogenesis of glaucoma. Advances in metabolomics technologies have allowed us to detect an increasing number of metabolites. Thus, metabolomic analysis of glaucomatous AH may provide potential biomarkers and expand our understanding of pathophysiology.
Ace Therapeutics can offer metabolomics technologies that can be used to study metabolic changes in glaucomatous AH and to investigate glaucoma biomarkers to better understand pathogenesis and identify new therapeutic strategies.
Metabolites of AH in Glaucoma
Fig. 1 Aqueous humor formation
causes elevated IOP in
glaucoma.
AH is a clear fluid present in the anterior part of the eye that provides nutrients to the tissues around the eye and eliminates metabolic waste products. Therefore, AH may be most closely associated with pathologic tissues and can provide us with physiopathologic information related to glaucoma. Metabolomics techniques offer a promising approach to the study of AH biospecimens, and advances in NMR and MS allow us to detect an increasing number of metabolites that can help us gain a comprehensive understanding of the molecular mechanisms of glaucoma.
AH contains a wide range of metabolites and more than 200 have been identified, including carbohydrates, amino acids, urea, ascorbic acid, glutathione organosolutes, adenosine, xanthine, polyamines, fatty acids, acylcarnitine, and phospholipids, involved in more than 40 metabolic pathways, including taurine metabolism, glycine, serine, and threonine metabolism, and alanine, aspartate, and glutamate metabolism.
Services for Metabolomic Profiling of Aqueous Humor in Glaucoma
Our service focuses on reporting metabolite analysis in glaucoma AH samples using advanced high-throughput metabolomics methods that can be applied to expand our understanding of molecular changes in glaucoma pathogenesis. A combination of targeted metabolomics techniques, untargeted metabolomics techniques and GC/TOF MS can be used to identify potential biomarkers associated with glaucoma and analyze the regulation of their metabolic pathways.
- Target samples
We specialize in glaucoma research, using animal models of glaucoma as subjects for metabolomic analysis of ocular biofluid AH. Collect AH samples from the eyes of experimental animals using a puncture technique with a fine needle. Ensure that there is little contact with other intraocular structures to prevent potential effects on metabolites in the AH samples. Centrifuge the samples to collect the supernatant and store at -80°C until metabolomic analysis. - Operation process
The main operational processes include metabolite extraction, separation and analysis, and data processing. Specific technical tools are available for clients to choose from, such as targeted metabolomics techniques, untargeted metabolomics techniques, targeted lipidomics techniques, untargeted lipidomics techniques, LC-MS, GC-MS, NMR. Compare the metabolomes of the control and test groups to determine the differences between their metabolite profiles. We also offer other metabolomics services not mentioned above, you can contact us for more. - Results delivery
You can obtain the relative quantitative values of specific metabolites in AH samples, metabolic pathway maps, and other results. The results of the analysis can only be used for research purposes. They cannot be used for other purposes, such as diagnosis. Analytical data will be saved after the analysis is completed and will be deleted at the end of the storage period. The price per sample can depend on the number of samples. You can contact us for more information on special pricing options.
Our Core Advantages
- State-of-the-art instrumentation for metabolomics analysis
- A professional research team focused on glaucoma
- Different service options from single analysis to collaborative projects
- Specializing in glaucoma-related services and research
For more information on how metabolomics can advance your glaucoma work, please contact us.
References
- Buisset A, et al. Metabolomic Profiling of Aqueous Humor in Glaucoma Points to Taurine and Spermine Deficiency: Findings from the Eye-D Study. Journal of Proteome Research, 2019, 18, 3, 1307-1315.
- Chen X, et al. Metabolomics of the aqueous humor in patients with primary congenital glaucoma. Molecular Vision, 2019, 25:489-501.