Ace Therapeutics provides a comprehensive and high-quality platform for oligonucleotide bioanalysis to our customers worldwide. We have a dedicated team in oligonucleotide bioanalysis with extensive experience in handling a wide range of issues during oligonucleotide bioanalysis. Therefore, you can be assured that your research project is in our hands.
The use of oligonucleotide drugs for the treatment of liver diseases is a very promising direction based on the inherent preference for oligonucleotide accumulation in the liver. Biological analysis of candidate oligonucleotide drugs is crucial due to the specificity of oligonucleotides. We have a full range of equipment and professional technicians to ensure the scientific and accurate analysis process. In addition, our scientists can design special analytical protocols for you based on the needs of your research project.
What Can We Do?
Oligonucleotides Bioanalysis Services
The bioanalysis protocols for oligonucleotides may vary depending on the experimental needs and the uniqueness of each oligonucleotide. Scientists at Ace Therapeutics will provide you with expert advice based on the uniqueness of your research project. Our experienced technical staff strictly controls all aspects of the bioanalytical process. Our scientists can also develop a unique bioanalytical protocol for you based on your needs.
- Oligonucleotide Purification
The synthesis process of oligonucleotides usually leads to the accumulation of impurities. Therefore, a purification step is essential before further analysis. We offer different purification methods to meet the needs of different research projects. Our scientists can also develop special purification protocols for you, depending on your experimental protocol.
- Oligonucleotides Bioanalysis
The length and chemical modification of oligonucleotides have a significant impact on the sensitivity of oligonucleotide bioanalytical assays due to the inherent polyanionic nature of oligonucleotides. We have a comprehensive analytical assay platform for different types of oligonucleotides. Also, our scientists will design exclusive oligonucleotide bioassay protocols for you based on different oligonucleotide molecules and different experimental protocols. Prior to the bioanalysis of oligonucleotides, a complex pre-processing of oligonucleotides is required. For this, we also provide high-quality and comprehensive services such as SPR purification, PCR amplification, and probe hybridization.
- In general, increasing the oligonucleotide length/size causes a decrease in the detection sensitivity of LC-MS/MS or LC-HRMS, but increases the sensitivity of hybridization-based assays (e.g., qPCR, LC-FL).
- For absolute quantitative bioanalysis of oligonucleotides, qPCR and LC-FL assays are more sensitive, while LC-MS/MS and LC-HRAM assays have better specificity.
- LC-MS is usually more suitable for the quantitative analysis of oligonucleotides containing 13 or fewer nucleotide units, while hybridization-based LC-FL analysis is more suitable for the quantitative analysis of oligonucleotides containing 24 or more nucleotide units
In addition, we can choose suitable analytical protocols according to the characteristics of oligonucleotide drugs.
- RT-qPCR will be mainly used for the quantification of unmodified oligonucleotides.
- For modified oligonucleotide drugs, LC-MS analytical method is preferred if the sensitivity meets the analytical needs.
- LC-HRMS of the Q-Exactive series will be chosen if the unknown metabolites need to be analyzed for identification and quantification.
- If both sensitivity and specificity are important and under conditions where LC can adequately separate oligonucleotides from their major metabolites, then LC-FL will be chosen.
Ace Therapeutics offers comprehensive services and technology platforms in oligonucleotide bioanalysis. Our thoughtful and comprehensive services are designed to facilitate the smooth conduct of your research projects. If you would like to learn more about our services, please feel free to contact us.
Our products and services are for research use only and can not be used for diagnostic or other purposes.
