Ace Therapeutics specializes in developing sophisticated liver fibrosis models, providing comprehensive support for disease mechanism research and drug development. Our customized models are meticulously designed to recapitulate the complex pathophysiology of liver fibrosis and are tailored to meet your specific research needs.
What Is Liver Fibrosis?
Liver fibrosis is a progressive disease characterized by excessive collagen accumulation, leading to liver scarring. It is primarily caused by chronic liver diseases such as hepatitis B and C, long-term alcohol abuse, non-alcoholic steatohepatitis (NASH), and autoimmune liver diseases. Pathologically, activated hepatic stellate cells produce large amounts of collagen, disrupting liver architecture and function, impairing regeneration, essential metabolic, detoxification, and synthetic processes.
Figure 1. Liver organoids are used to establish liver fibrosis models for the study of liver fibrosis mechanisms and drug development and screening (Bao Y.L., et al., 2021)
Our In Vitro Models of Liver Fibrosis
Primary hepatic stellate cell (HSC) isolation and culture
- We offer HSCs in different activation states to study the pathogenesis.
HSC cell lines
- Cell lines such as LX-2 offer advantages for genetic manipulation studies and high-throughput screening.
iPSC-derived HSCs
- Our customized services help develop iPSC-HSCs that closely mimic primary cells for mechanistic research and drug development.
HSC co-culture systems
- We can set up co-cultures of HSCs with hepatocytes and other liver cells to mimic cell-cell interactions and the tissue microenvironment that drive fibrosis progression.
3D liver spheroids and organoids
- We specialize in 3D spheroid culture and organoid model construction for liver fibrosis, offering comprehensive services to support advanced research and drug development in this field.
Microfluidic liver chips
- We can engineer liver microfluidic chips to simulate the complex liver microenvironment, offering a powerful tool for studying liver function and disease progression in a controlled and dynamic environment.
How Do We Customize Animal Models of Liver Fibrosis?
| Model Types |
Animal Species |
Modeling Methods |
| Chemically induced models |
- Rat
- Mouse
- Macaque
- Marmoset
|
- Alcohol-induced liver fibrosis models
- CCl4-induced liver fibrosis models
- TAA-induced liver fibrosis models
- Nitrosamines-induced liver fibrosis models (DMN, DEN)
- Liver toxins-induced liver fibrosis models (arsenic, APAP, D-GalN)
|
| Diet induced models |
|
- Choline-deficient diet (MCD)-induced liver fibrosis models
- High-fat diet (HFD)-induced liver fibrosis models
- Western diet (WD)-induced liver fibrosis models
- CDAA-induced liver fibrosis model
- CDAHFD-induced liver fibrosis model
|
| Surgical animal models |
|
|
| Transgenic animal models |
|
- Gnmt-deficient (Gnmt) mice
- Liver-specific O-GlcNAc transferase-KO mice
- Mdr2−/− mice
|
| Immune induction methods |
|
- Schistosoma-induced liver fibrosis model
- Virus-induced liver fibrosis model
- Porcine serum (PS)-induced liver fibrosis model
- Concanavalin A-induced liver fibrosis model
|
| Combine induction methods |
|
- Chemical & Chemical (CCl4 & Ethanol)
- Chemical & Diet (HFD & Ethanol, CCl4 & WD, TAA & FFD)
- Transgenic & Diet (ob/ob & HFD, adropin-KO & MCD, adropin-KO & WD)
|
By leveraging our tailored liver fibrosis modeling services, Ace Therapeutics provides researchers with a holistic approach to gain valuable insights and expedite their investigations. Whether you need efficacy evaluation services for candidates or targeted pathway analysis, our team is committed to collaborating closely with you to craft personalized solutions aligned with your precise research goals. Contact us today to discover how our services can streamline your project timelines.
References
- Bao Y.L., et al. Animal and organoid models of liver fibrosis. Front Physiol. 2021, 12:666138.
- Wu S., et al. An update on animal models of liver fibrosis. Front Med (Lausanne). 2023, 10:1160053.
Related Services
Our products and services are for research use only and can not be used for diagnostic or other purposes.
