Disease Models

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* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Custom Gastroesophageal Reflux Disease (GERD) Models

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At Ace Therapeutics, we understand the importance of accurate and reliable disease models to advance research and drug development in digestive health. Our expertise lies in tailoring disease models to mimic specific conditions, such as gastroesophageal reflux disease (GERD). Our GERD model customization services encompass the development of animal models that recapitulate key aspects of GERD pathology, including esophageal inflammation, mucosal damage, reflux episodes, and acid secretion dysregulation.

What Is Gastroesophageal Reflux Disease?

Gastroesophageal reflux disease is a chronic digestive disorder characterized by the backward flow of stomach acid and contents into the esophagus. This condition can lead to symptoms such as heartburn, regurgitation, chest pain, and inflammation of the esophageal lining.

Figure 1. Multifactorial pathophysiology of GERD (Argüero J.; Sifrim D, 2024)

What Can We Do for Gastroesophageal Reflux Disease Model Customization?

In Vitro GERD Model Customization Services

Cell Culture Models: Establishing in vitro models using esophageal epithelial cells, smooth muscle cells, and immune cells to study cellular responses to acid exposure, inflammation, and barrier dysfunction.
Organoid Cultures: Developing 3D organoid models of the esophagus to investigate tissue architecture, cell interactions, and responses to GERD.
Co-culture Systems: Creating co-culture systems involving epithelial cells, fibroblasts, and immune cells to simulate the complex interactions involved in GERD pathogenesis and progression.
Functional Assays: Performing functional assays to assess mucosal integrity, acid secretion, motility patterns, cytokine release, and gene expression changes in response to GERD stimuli.

In Vivo GERD Model Customization Services

Animal Model Selection: Tailor-made selection of appropriate animal species (e.g., rodents, pigs) based on research objectives and desired physiological similarities to human GERD.
GERD Induction: Development of GERD models through surgical procedures (e.g., esophagogastric anastomosis, pyloric ligation) or chemical induction methods (e.g., acid infusion, use of irritants).
Model Validation: Evaluating GERD symptoms such as acid reflux, esophageal inflammation, mucosal damage, regurgitation, and altered motility patterns.
Monitoring and Assessment: Implementing monitoring protocols for evaluating reflux episodes, pH levels in the esophagus, histological changes, inflammatory markers, and behavioral indicators.

Our Platforms for Gastroesophageal Reflux Disease Model Customization

Technologies	Platforms	Services Introduction
Imaging Technologies	High-resolution endoscopy	Detailed visualization of esophageal mucosa, reflux episodes, and inflammatory changes.
	Fluorescence imaging	Assesses cellular changes, mucosal integrity, and barrier function in GERD models.
	Microscopic imaging	Provides detailed histological analysis of tissue samples, identifying microscopic features of GERD pathology.
Physiological Monitoring Systems	pH monitoring	Continuous monitoring of esophageal pH to track acid reflux episodes and assess the severity of reflux.
	Manometry	Measures esophageal motility patterns, sphincter function, and gastric contractions critical for GERD model characterization.
	Impedance testing	Evaluates bolus movement, reflux events, and esophageal clearance, providing insights into GERD-related motility disorders.
Molecular Analysis Techniques	Gene expression profiling	Utilizes RNA sequencing, qPCR, and microarray analysis to assess gene expression changes in GERD models, identifying key molecular pathways.
	Proteomics	Identifies and quantifies proteins involved in GERD pathogenesis, inflammation, and tissue damage using techniques such as western blotting and mass spectrometry.
	Immunohistochemistry	Localizes specific proteins, markers of inflammation, and cell types within GERD-affected tissues, aiding in histological characterization.
Pharmacological Testing	Pharmacodynamic studies	Tests potential therapeutic compounds, acid suppressants, prokinetics, and mucosal protectants for their efficacy in alleviating GERD symptoms in preclinical models.

At Ace Therapeutics, we offer comprehensive and advanced gastroesophageal reflux disease (GERD) model customization services designed to meet the diverse needs of researchers and pharmaceutical companies. Researchers and organizations looking to advance their GERD research and drug development efforts are invited to inquire about our customizable GERD models, tailored to their specific research objectives and requirements. Contact us today to learn more and collaborate on solutions for GERD research.

Reference

Argüero J., Sifrim D. Pathophysiology of gastro-oesophageal reflux disease: implications for diagnosis and management. Nat Rev Gastroenterol Hepatol. 2024, 21(4):282-293.

Our products and services are for research use only and can not be used for diagnostic or other purposes.