Ace Therapeutics specializes in providing disease models and customized services for the study of esophageal diseases. Our disease models can mimic the complex biology of esophageal diseases, enabling researchers to gain valuable insights into disease mechanisms and evaluate the efficacy of potential therapeutics.
What Esophageal Diseases Can We Model?
- Esophageal Stricture: Tailor disease models to replicate the characteristics and progression of esophageal strictures for enhanced understanding and therapeutic interventions.
- Reflux Esophagitis: Develop customized disease models that mimic reflux esophagitis, enabling in-depth research and the evaluation of novel treatment strategies.
- Esophageal Cancer: Create disease models that closely resemble esophageal cancer, facilitating targeted investigations and the development of effective diagnostic and therapeutic approaches.
What Types of Esophageal Disease Models Can We Customize?
We can engineer cells to mimic disease microenvironments, allowing us to help customers study disease mechanisms and evaluate drug responses. One example is using CRISPR/Cas9 to introduce disease-related mutations into esophageal epithelial cells and fibroblasts. We also specialize in inducing pluripotent cells or tissue stem cells to differentiate specifically into target lineages such as Barrett's columnar epithelium through small molecule cocktails.
- Esophageal Organoid Models
Suitable tissues or cells, such as somatic cells, adult stem cells, or pluripotent stem cells, are collected from humans or animals. Depending on the specific organoid type, we employ scaffold-based or scaffold-free 3D cell culture methods, adjusting the composition of the culture medium and necessary cytokines to support organoid growth.
Esophageal Stricture Animal Model Customization
| Animal Species |
- SPF grade Balb/C mouse, healthy, male, 4~6 weeks, body weight 18 g~20 g
|
| Modeling Method |
- Our expertise includes electrothermal biopsy forceps cauterization under gastroscopy to induce stenosis, allowing observation of mucosal changes and pathological analysis.
- Our models replicate esophageal stenosis progression, from congestion and edema to the formation of ulcers, fibrosis, and scar.
|
| Animal Model Validation |
- Our scientists can perform gastroscopy evaluations, tissue sampling, and pathological analysis to examine epithelial changes, ulcer development, hyperplasia, granulation formation, and inflammatory responses.
|
Reflux Esophagitis Animal Model Customization
| Animal Species |
- SD/Wistar rats, 6~8 weeks, male.
|
| Modeling Method |
- Ace Therapeutics can develop customized esophageal disease models through surgical techniques. Our scientists are trained in creating esophageal-duodenal anastomoses in rodents, mimicking conditions like Barrett's esophagus.
|
| Animal Model Validation |
- Through H&E staining experiment, we examine esophageal mucosal morphology and inflammatory responses.
- Post-operative monitoring of diet, activity and mental status also ensures model welfare and validity.
|
Esophageal Cancer Animal Model Customization
| Animal Species |
|
| Modeling Methods |
- Induced Animal Models: 4-NQO (4-nitroquinoline-1-oxide) and nitrosamine compounds are commonly used carcinogenic mutagens in our experimental animals, and the most commonly used are NMBA and MNAN/AMN.
- PDX Models: Human esophageal cancer cell lines or esophageal cancer tissue blocks are directly transplanted into the subcutaneous tissue of immunodeficient mice to establish models. Our commonly used human esophageal cancer cell lines include EC109, KYSE510, KYSE150, KYSE30, TE-1 and TE-5.
- Transgenic Animal Models: We can use genetic engineering technology, such as CRISPR/Cas9 technology, to transfer or knock out genes to construct transgenic esophageal cancer animal models. Common esophageal cancer-related genes include cyclin D1, p53, P120-Catenin, and Brcal.
|
| Animal Model Validation |
- These models will undergo thorough examination using histopathological analysis, molecular profiling, and tumor growth monitoring, ensuring their fidelity and relevance for esophageal cancer research.
|
What Can We Do for Preclinical Esophageal Disease Research?
- Disease Mechanism Studies
Our models allow for in-depth investigations into the underlying mechanisms of esophageal diseases, facilitating a better understanding of disease progression and identifying potential therapeutic targets.
We can utilize our models to help you screen and evaluate the efficacy of potential drugs and interventions for esophageal diseases, aiding in the identification of promising candidates for further development.
Our models help in the identification and validation of biomarkers associated with esophageal diseases, enabling early detection, prognosis assessment, and monitoring of disease progression.
Comprehensive data sets are collected from model experimentation, including omics readouts, imaging outputs and functional readouts. We offer valuable insights into treatment responses and strategies.
Ace Therapeutics specializes in developing disease models tailored to your research needs. With robust validation, diverse model options, and ongoing scientific support, we can accelerate your esophageal disease research. Contact us today to inquire about our customizable disease models and propel your research forward!
Related Products
 |
Esophageal cell lines such as EC109, KYSE510, KYSE150, KYSE30, and TE-1 |
Inquiry |
 |
Esophageal Cancer Organoid Culture Kit |
Inquiry |
Related Services
Our products and services are for research use only and can not be used for diagnostic or other purposes.
