Ace Therapeutics specializes in the development of customized disease models for appendicitis, enabling the in-depth study of disease mechanisms and facilitating the development of novel drugs and diagnostic technologies. With our expertise and cutting-edge methodologies, we provide comprehensive support to researchers and pharmaceutical companies in their quest to understand and combat appendicitis.
Pathological Features of Appendicitis
Appendicitis is characterized by inflammation of the appendix, typically caused by blockage of the appendix opening. Pathologically, it exhibits features such as neutrophil infiltration, mucosal ulceration, and possible abscess formation.
Figure 1. Histopathological features of the vermiform appendix (Reismann M., 2022)
How Do We Customize Appendicitis Models?
Ace Therapeutics offers highly customized appendicitis models in rats and rabbits, utilizing sophisticated techniques to ensure accurate replication of pathology. Our models provide valuable insights into disease mechanisms and treatment efficacy, supporting the development of innovative drugs and diagnostic technologies. With meticulous attention to detail and adherence to aseptic principles, we guarantee reliable and relevant research outcomes.
| Animal Species |
Advantages |
| Rats |
- We utilize adult rats to create appendicitis models due to their manageable size and well-understood physiology.
- Rat models are ideal for preliminary studies and high-throughput screening of therapeutic interventions.
|
| Rabbits |
- Adult rabbits are chosen for their anatomical and physiological similarities to humans, offering a closer approximation of clinical appendicitis.
|
- Methods for Constructing Appendicitis Models
| Methods |
Service Details |
| Bacterial Infection Modeling |
With our expertise, we can introduce bacterial infections directly into the appendix or peritoneal cavity, utilizing bacterial species known to cause appendicitis, such as E. coli or B. fragilis. |
| Obstruction Modeling |
We employ surgical or mechanical techniques to occlude the appendiceal lumen in organoids or animals. The obstruction prevents the normal flow of contents, leading to accumulation, inflammation, and subsequent development of appendicitis-like symptoms. |
- Model Validation & Drug Efficacy Evaluation
Behavioral Monitoring
Pathological Examination
Imaging
Biochemical and Microbiological Tests
- Monitor animal behavior and physical condition, noting symptoms such as listlessness, abdominal bloating, and refusal to eat or drink.
- Record body temperature regularly to detect fever, which indicates infection and inflammation.
- Conduct post-mortem examinations to observe the appendix for signs of congestion, edema, purulent exudate, and necrosis.
- Perform histological analysis to confirm tissue changes associated with appendicitis, such as inflammatory cell infiltration and fibrous cyst formation.
- Use ultrasound or CT imaging to visualize the appendix and surrounding tissues, confirming structural changes and inflammation.
- Measure inflammatory markers, such as cytokine levels, in blood samples to evaluate the effect of drugs on systemic inflammation.
- Culture the contents of the necrotic appendicitis cavity to identify bacterial pathogens.
- Culture bacterial samples from the appendix and peritoneal fluid to assess any reduction in bacterial load due to the drug.
At Ace Therapeutics, we offer custom appendicitis model services tailored to your specific research needs. We can help partners test the efficacy of novel therapies, such as inflammation reduction, infection clearance, and symptom improvement. If your organization seeks a streamlined approach to exploring appendicitis mechanisms or evaluating new treatments, please contact us to discuss our modeling capabilities.
Reference
- Reismann M. A concise pathophysiological model of acute appendicitis against the background of the COVID-19 pandemic. Front Pediatr. 2022, 10:908524.
Related Services
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