Cat. No.: DAA-001065
| Product Information | |
|---|---|
| Product Name | Total SQSTM1/p62 Sandwich ELISA Kit |
| Species | Human; Monkey |
| Description | The Total SQSTM1/p62 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of SQSTM1/p62 protein. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with SQSTM1/p62 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of SQSTM1/p62. |
| Component | SQSTM1/p62 Mouse mAb Coated Microwells; SQSTM1/p62 Rabbit Detection mAb; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | ATG4A |
| UniProt No. | Q8WYN0 |
| Gene ID | 115201 |
| Target Description | Sequestosome 1. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. Protein aggregates formed by SQSTM1 can be degraded by the autophagosome. SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome. Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| Alternative Names | AUT-like 2 cysteine endopeptidase; Autophagy-related cysteine endopeptidase (Autophagin-2); Autophagy-related protein 4 homolog A (HsAPG4A; hAPG4A) |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
