Cat. No.: DAA-001128
| Product Information | |
|---|---|
| Product Name | Total HIF-1α Sandwich ELISA Kit |
| Species | Human |
| Description | The Total HIF-1α Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total HIF-1α protein. A HIF-1α antibody has been coated onto the microwells. After incubation with cell lysates, HIF-1α protein is captured by the coated antibody. Following extensive washing, an HIF-1α Detection Antibody is added to detect the captured HIF-1α protein. Anti-Rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of HIF-1α protein. |
| Component | HIF-1α Goat Antibody Coated Microwells; HIF-1α Rabbit Detection mAb; Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | HIF1A |
| UniProt No. | Q16665 |
| Gene ID | 3091 |
| Target Description | Hypoxia-inducible factor 1.HIF-1β is also known as AhR nuclear translocator. |
| Alternative Names | ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS protein 1; PAS domain-containing protein 8 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
