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Cat. No.: DAA-001123
| Product Information | |
|---|---|
| Product Name | Total GSK-3β Sandwich ELISA Kit |
| Species | Human; Mouse; Rat; Monkey |
| Description | The Total GSK-3β Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of GSK-3β protein. A GSK-3β mouse mAb has been coated onto the microwells. After incubation with cell lysates, GSK-3β (phospho and nonphospho) protein is captured by the coated antibody. Following extensive washing, a GSK-3β rabbit mAb is added to detect the captured GSK-3β protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total GSK-3β. |
| Component | GSK-3β Mouse mAb Coated Microwells; GSK-3β Rabbit Detection mAb; Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | GSK3B |
| UniProt No. | P49841 |
| Gene ID | 2932 |
| Target Description | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization. |
| Alternative Names | Glycogen Synthase Kinase 3β; Glycogen synthase kinase-3β; GSK 3β; GSK-3β; GSK3B; GSK3B_HUMAN; GSK3β isoform; Serine/threonine-protein kinase GSK3B |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
