x
- Home
- About Diabetes
- Services
- Diabetes Mellitus Modeling Solutions
- R&D Services for Diabetes Mellitus and Diabetic Complications
- By Drug Development Process
- About Us
- Careers
- Contact Us
Cat. No.: DAA-001230
| Product Information | |
|---|---|
| Product Name | Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) |
| Description | The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) uses the β-gal Substrate 4-Methylumbelliferyl β-D-galactopyranoside (4-MUG) to detect Senescence-Associatedβ-galactosidase (SA-β-gal) activity. Upon binding to β-gal, 4-MUG is hydrolysed to the fluorescent product 4-MU that can be measured at an excitation wavelength of 360 nm and an emission wavelength of 465 nm. Fluorescent intensity correlates with sample β-gal levels. This quantitative assay uses cellular lysates for determination of SA-β-gal activity. Each kit provides enough reagents to perform up to 100 assays in a 96-well plate format. |
| Component | Senescence 1X Cell Lysis Buffer; Senescence 2X Reaction Buffer; Senescence Stop Solution; SA-β-Gal Substrate |
| Target Information | |
|---|---|
| Target Name | lacZ |
| UniProt No. | P00722 |
| Gene ID | 945006 |
| Target Description | β-galactosidase, defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells, SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence. |
| Alternative Names | Lactase; β-gal |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
