Cat. No.: DAA-001230
| Product Information | |
|---|---|
| Product Name | Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) |
| Description | The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) uses the β-gal Substrate 4-Methylumbelliferyl β-D-galactopyranoside (4-MUG) to detect Senescence-Associatedβ-galactosidase (SA-β-gal) activity. Upon binding to β-gal, 4-MUG is hydrolysed to the fluorescent product 4-MU that can be measured at an excitation wavelength of 360 nm and an emission wavelength of 465 nm. Fluorescent intensity correlates with sample β-gal levels. This quantitative assay uses cellular lysates for determination of SA-β-gal activity. Each kit provides enough reagents to perform up to 100 assays in a 96-well plate format. |
| Component | Senescence 1X Cell Lysis Buffer; Senescence 2X Reaction Buffer; Senescence Stop Solution; SA-β-Gal Substrate |
| Target Information | |
|---|---|
| Target Name | lacZ |
| UniProt No. | P00722 |
| Gene ID | 945006 |
| Target Description | β-galactosidase, defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells, SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence. |
| Alternative Names | Lactase; β-gal |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
