Cat. No.: DAA-001069
| Product Information | |
|---|---|
| Product Name | Rat BDNF ELISA Kit |
| Species | Rat |
| Description | The Rat BDNF Enzyme-Linked Immunosorbent Assay (ELISA) kit is designed for the quantitative measurement of Rat BDNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BDNF has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat BDNF/CFH amount of sample captured in plate. |
| Sample Types | Cell Culture Supernatant, Serum, Cell Lysate, Hep Plasma, Edta Plasma, Cit Plasma |
| Detection Range | 31.2 pg/mL - 2000 pg/mL |
| Sensitivity | < 15 pg/mL |
| Target Information | |
|---|---|
| Target Name | BDNF |
| UniProt No. | P23560 |
| Gene ID | 627 |
| Target Description | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation, long-term depression, certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. |
| Alternative Names | Abrineurin; ANON2; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
