Rabbit TREM2 Signaling Pathways Sampler Kit Monoclonal Antibody

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Cat. No.: DAB-0012926

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Monoclonal
Host Species	Rabbit
Product Description	Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues around Leu221 of human TREM2 protein, near the carboxy terminus of human Dap12, around Asn463 of human Syk protein, around Leu1220 of human PLCγ1, and near the amino terminus of human PLCγ2. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Tyr525/526 of human Syk, Tyr352 of human Syk, Tyr759 of human PLCγ2, and Tyr783 of human PLCγ1.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	Microglia cells are resident macrophages of the brain that survey the brain environment and dynamically respond to maintain brain homeostasis. Microglial responses include phagocytosis of cellular debris, restricting sites injury or pathology, and/or releasing inflammatory signals to initiate an immune response. Such responses are important during normal development and during diseased states.Recently, the role of microglia in neurodegenerative disease pathology, particularly Alzheimer’s disease, has been of intense investigation. Much of this work is driven by human genetic data that links microglia-enriched genes with AD progression. The triggering receptor expressed on myeloid cells 2 protein is an innate immune receptor that is expressed on the cell surface of microglia. TREM2 plays a role in innate immunity, and a rare functional variant of the TREM2 gene is associated with the late-onset risk of AD. How TREM2 contributes to disease function is currently an active area of research, but might drive a number of microglial cellular functions ranging from microgliosis, phagocytosis, and cytokine release via a variety of signaling cascades triggered by TREM2.The TREM2 receptor is a single-pass type I membrane glycoprotein that consists of an extracellular immunoglobulin-like domain, a transmembrane domain, and a cytoplasmic tail. Ligands for TREM2 include phospholipids, apolipoproteins, and lipoproteins. Upon activation, TREM2 interacts with the tyrosine kinase-binding protein DNAX-activating protein 12 to form a receptor-signaling complex. Ligand binding by DAP12-associated receptors, including TREM2, results in phosphorylation of tyrosine residues within the DAP12 immunoreceptor tyrosine-based activation motif by Src family kinases; ITAM phosphorylation leads to activation of spleen tyrosine kinase and downstream signaling cascades. Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain and phosphorylation at these residues is essential for Syk function. Syk phosphorylation is also a readout for β-amyloid triggered TREM2 activity. Phosphoinositide-specific phospholipase C γ 1/2 is reported to be down stream of Syk. Tyr352 of Syk is involved in the association of PLCγ1; Syk-mediated phosphorylation PLCγ1 at Tyr783 activates PLCγ1 enzymatic activity. Interestingly, mutations in the microglia-enriched PLCγ2 gene are associated with AD.

Target Information

Gene Description

Microglia cells are resident macrophages of the brain that survey the brain environment and dynamically respond to maintain brain homeostasis. Microglial responses include phagocytosis of cellular debris, restricting sites injury or pathology, and/or releasing inflammatory signals to initiate an immune response. Such responses are important during normal development and during diseased states.Recently, the role of microglia in neurodegenerative disease pathology, particularly Alzheimer’s disease, has been of intense investigation. Much of this work is driven by human genetic data that links microglia-enriched genes with AD progression. The triggering receptor expressed on myeloid cells 2 protein is an innate immune receptor that is expressed on the cell surface of microglia. TREM2 plays a role in innate immunity, and a rare functional variant of the TREM2 gene is associated with the late-onset risk of AD. How TREM2 contributes to disease function is currently an active area of research, but might drive a number of microglial cellular functions ranging from microgliosis, phagocytosis, and cytokine release via a variety of signaling cascades triggered by TREM2.The TREM2 receptor is a single-pass type I membrane glycoprotein that consists of an extracellular immunoglobulin-like domain, a transmembrane domain, and a cytoplasmic tail. Ligands for TREM2 include phospholipids, apolipoproteins, and lipoproteins. Upon activation, TREM2 interacts with the tyrosine kinase-binding protein DNAX-activating protein 12 to form a receptor-signaling complex. Ligand binding by DAP12-associated receptors, including TREM2, results in phosphorylation of tyrosine residues within the DAP12 immunoreceptor tyrosine-based activation motif by Src family kinases; ITAM phosphorylation leads to activation of spleen tyrosine kinase and downstream signaling cascades. Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain and phosphorylation at these residues is essential for Syk function. Syk phosphorylation is also a readout for β-amyloid triggered TREM2 activity. Phosphoinositide-specific phospholipase C γ 1/2 is reported to be down stream of Syk. Tyr352 of Syk is involved in the association of PLCγ1; Syk-mediated phosphorylation PLCγ1 at Tyr783 activates PLCγ1 enzymatic activity. Interestingly, mutations in the microglia-enriched PLCγ2 gene are associated with AD.

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at +4 °C short term (1-2 weeks). Store at -20 °C long term.
Storage Buffer	Constituent: 100% PBS

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