Rabbit TREM2-dependent mTOR Metabolic Fitness Sampler Kit Monoclonal Antibody

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Cat. No.: DAB-0012927

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Monoclonal
Host Species	Rabbit
Product Description	Monoclonal antibodies to total proteins are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu221 of human TREM2 protein, Arg21 of human AMPKα protein, Ser2481 of human mTOR protein, Gly215 of mouse TREM2 protein, Leu44 of human LC3B protein (conserved in LC3A), and the carboxy-terminal sequence of mouse Akt protein. Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr172 of human AMPKα protein, Ser2448 of human mTOR protein, and Ser473 of human Akt protein.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	The triggering receptor expressed on myeloid cells 2 protein is an innate immune receptor that is expressed on the cell surface of microglia, macrophages, osteoclasts, and immature dendritic cells. The TREM2 protein plays a role in innate immunity and a rare functional variant of TREM2 is associated with the late-onset risk of Alzheimer’s disease . Research studies using mouse models of AD indicate that deficiency and haploinsufficiency of TREM2 can lead to increased β-amyloid accumulation as a result of dysfunctional microglia response. Activation of TREM2 in mouse models of AD ameliorates several forms of AD pathology, likely through a microglia-specific mechanism. This mechanism is under intense investigation, but may involve TREM2-dependent maintenance microglia energetic and biosynthetic metabolism. Autophagy is one mechanism by which cellular metabolism is maintained and, in the absence of TREM2, several AMPK-dependent autophagy cell signaling pathways are enhanced. AMP-activated protein kinase is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation. AMPK is further regulated by several proteins within a regulatory cell signaling pathway. The mammalian target of rapamycin is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. Akt, also referred to as PKB or Rac, is regulated by phosphorylation at Ser473. The presence of autophagy marker Light Chain 3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy.

Target Information

Gene Description

The triggering receptor expressed on myeloid cells 2 protein is an innate immune receptor that is expressed on the cell surface of microglia, macrophages, osteoclasts, and immature dendritic cells. The TREM2 protein plays a role in innate immunity and a rare functional variant of TREM2 is associated with the late-onset risk of Alzheimer’s disease . Research studies using mouse models of AD indicate that deficiency and haploinsufficiency of TREM2 can lead to increased β-amyloid accumulation as a result of dysfunctional microglia response. Activation of TREM2 in mouse models of AD ameliorates several forms of AD pathology, likely through a microglia-specific mechanism. This mechanism is under intense investigation, but may involve TREM2-dependent maintenance microglia energetic and biosynthetic metabolism. Autophagy is one mechanism by which cellular metabolism is maintained and, in the absence of TREM2, several AMPK-dependent autophagy cell signaling pathways are enhanced. AMP-activated protein kinase is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation. AMPK is further regulated by several proteins within a regulatory cell signaling pathway. The mammalian target of rapamycin is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. Akt, also referred to as PKB or Rac, is regulated by phosphorylation at Ser473. The presence of autophagy marker Light Chain 3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy.

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at +4 °C short term (1-2 weeks). Store at -20 °C long term.
Storage Buffer	Constituent: 100% PBS

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