Rabbit PKA C-α Polyclonal Antibody

Rabbit PKA C-α Polyclonal Antibody

Cat. No.: DAB-0012704

Size: 20 µL Size: 100 µL Size: Customer Size
Product Information
Clonality Polyclonal
Host Species Rabbit
Reactivity Human, Mouse, Rat
Applications WB, IP, IF, Flow Cyt
Product Description Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminal sequence of human PKA C-α. Antibodies are purified by protein A and peptide affinity chromatography.
Format Liquid
Purity Affinity purity
Target Information
Target Name PRKACA
UniProt No. P17612
Gene ID 5566
Gene Description The second messenger cyclic AMP activates cAMP-dependent protein kinase in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation. Inactive PKA is a heterotetramer composed of a regulatory subunit dimer and a catalytic subunit dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms and two families of regulatory subunits with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β. Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue. Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad, CREB, and GSK-3. In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf. Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197.
Shipping & Storage
Shipping Shipped at 4 °C.
Storage Instructions Store at –20 °C. Do not aliquot the antibody.
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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