Rabbit One-Carbon Metabolism Sampler Kit Monoclonal Antibody

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Cat. No.: DAB-0012371

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Monoclonal
Host Species	Rabbit
Product Description	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human AHCYL1/IRBIT protein, Leu220 of human CBS protein, Pro298 of human cystathionine γ-lyase protein, Pro39 of human MTHFR protein, Ala264 of human MTHFD1L protein, Leu340 of human MTHFD2 protein, Glu187 of human SHMT1 protein, and residues near the carboxy terminus of human thymidylate synthase protein.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	One-carbon metabolism includes various enzymatic reactions involving the transfer of one-carbon groups mediated by folate cofactor. The activated one-carbon groups are used by various metabolic pathways, including purine synthesis, thymidine synthesis, and remethylation of homocysteine to methionine. S-adenosylhomocysteine hydrolase-like protein 1 is a member of the S-adenosylhomocysteine hydrolase family, which participates in the metabolism of S-adenosyl-L-homocysteine. Cystathionine beta-synthase is a key enzyme involved in sulfur amino acid metabolism as it catalyzes the formation of cystathionine from serine and homocysteine. Cystathionine γ-lyase is an enzyme in the transsulfuration pathway, a route in the metabolism of sulfur-containing amino acids. Methylenetetrahydrofolate reductase, a key enzyme in one-carbon metabolism, catalyzes the conversion of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate. 5-methyltetrahydrofolate donates its methyl group for remethylation of homocysteine to methionine. Methionine is further converted to S-adenosylmethionine, a major reactive methyl carrier. NADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate in one-carbon flow from mitochondria to cytoplasm. MTHFD2 is a bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase involved in mitochondrial folate metabolism. Serine hydroxymethyltransferase 1 is a cytoplasmic serine hydroxylmethyltransferase. It catalyzes the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate to form 5, 10-methylene-THF. The methylation of deoxyuridine monophosphate to deoxythymidine monophosphate is an essential step in the formation of thymine nucleotides, a process catalyzed by thymidylate synthase .

Target Information

Gene Description

One-carbon metabolism includes various enzymatic reactions involving the transfer of one-carbon groups mediated by folate cofactor. The activated one-carbon groups are used by various metabolic pathways, including purine synthesis, thymidine synthesis, and remethylation of homocysteine to methionine. S-adenosylhomocysteine hydrolase-like protein 1 is a member of the S-adenosylhomocysteine hydrolase family, which participates in the metabolism of S-adenosyl-L-homocysteine. Cystathionine beta-synthase is a key enzyme involved in sulfur amino acid metabolism as it catalyzes the formation of cystathionine from serine and homocysteine. Cystathionine γ-lyase is an enzyme in the transsulfuration pathway, a route in the metabolism of sulfur-containing amino acids. Methylenetetrahydrofolate reductase, a key enzyme in one-carbon metabolism, catalyzes the conversion of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate. 5-methyltetrahydrofolate donates its methyl group for remethylation of homocysteine to methionine. Methionine is further converted to S-adenosylmethionine, a major reactive methyl carrier. NADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate in one-carbon flow from mitochondria to cytoplasm. MTHFD2 is a bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase involved in mitochondrial folate metabolism. Serine hydroxymethyltransferase 1 is a cytoplasmic serine hydroxylmethyltransferase. It catalyzes the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate to form 5, 10-methylene-THF. The methylation of deoxyuridine monophosphate to deoxythymidine monophosphate is an essential step in the formation of thymine nucleotides, a process catalyzed by thymidylate synthase .

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at +4 °C short term (1-2 weeks). Store at -20 °C long term.
Storage Buffer	Constituent: 100% PBS

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