Rabbit mTOR Pathway Sampler Kit Polyclonal Antibody

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Cat. No.: DAB-0012320

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Polyclonal
Host Species	Rabbit
Product Description	Polyclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2481 of human mTOR. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR, Gln1681 of human Rictor, Gln210 of human GβL and human Raptor.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	The mammalian target of rapamycin is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy.The regulatory associated protein of mTOR interacts with mTOR to mediate mTOR signaling to downstream targets. Raptor binds to mTOR substrates, such as 4E-BP1 and p70 S6 kinase, through their TOR signaling motifs and is required for mTOR-mediated substrate phosphorylation. Binding of the FKBP12-rapamycin complex to mTOR inhibits mTOR-raptor interaction, which suggests a mechanism for the inhibition of mTOR signaling by rapamycin. This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL. GβL is part of the rapamycin-insensitive complex between mTOR and rictor and may mediate rictor-mTOR signaling to PKCα and other downstream targets. The rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB at Ser473, which is required for PDK1 phosphorylation of Akt/PKB at Thr308 and full activation of Akt/PKB.

Target Information

Gene Description

The mammalian target of rapamycin is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy.The regulatory associated protein of mTOR interacts with mTOR to mediate mTOR signaling to downstream targets. Raptor binds to mTOR substrates, such as 4E-BP1 and p70 S6 kinase, through their TOR signaling motifs and is required for mTOR-mediated substrate phosphorylation. Binding of the FKBP12-rapamycin complex to mTOR inhibits mTOR-raptor interaction, which suggests a mechanism for the inhibition of mTOR signaling by rapamycin. This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL. GβL is part of the rapamycin-insensitive complex between mTOR and rictor and may mediate rictor-mTOR signaling to PKCα and other downstream targets. The rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB at Ser473, which is required for PDK1 phosphorylation of Akt/PKB at Thr308 and full activation of Akt/PKB.

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at +4 °C short term (1-2 weeks). Store at -20 °C long term.
Storage Buffer	Constituent: 100% PBS

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