Rabbit LDHA/LDHC (C28H7) Monoclonal Antibody

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Cat. No.: DAB-0012237

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Monoclonal
Isotype	IgG
Host Species	Rabbit
Reactivity	Human, Mouse, Rat, Monkey
Applications	WB, IHC
Product Description	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	Lactate dehydrogenase catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A isozyme. The LDHA promoter contains HIF-1α binding sites. LDHA expression is induced under hypoxic conditions. During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.While LDHA is found primarily in muscle and kidney, LDHC is a testis-specific isoform. Both proteins are associated with human disease. Mutations in the corresponding LDHA gene are associated with LDHA deficiency, which is characterized by muscle stiffness following exercise and uterine stiffness during pregnancy. Abnormal LDHC expression is associated with several forms of cancer.

Target Information

Gene Description

Lactate dehydrogenase catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A isozyme. The LDHA promoter contains HIF-1α binding sites. LDHA expression is induced under hypoxic conditions. During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.While LDHA is found primarily in muscle and kidney, LDHC is a testis-specific isoform. Both proteins are associated with human disease. Mutations in the corresponding LDHA gene are associated with LDHA deficiency, which is characterized by muscle stiffness following exercise and uterine stiffness during pregnancy. Abnormal LDHC expression is associated with several forms of cancer.

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at –20 °C. Do not aliquot the antibody.
Storage Buffer	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

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